FcRn Overexpression Expands Diversity of the Humoral Immune Response in bFcRn Transgenic Mice.
Identifieur interne : 000149 ( Main/Exploration ); précédent : 000148; suivant : 000150FcRn Overexpression Expands Diversity of the Humoral Immune Response in bFcRn Transgenic Mice.
Auteurs : Bence Szikora [Hongrie] ; Anita Marx [Hongrie] ; Péter K. Jani [Hongrie] ; Orsolya Pipek [Hongrie] ; Viktor Müller [Hongrie] ; István Csabai [Hongrie] ; Imre Kacskovics [Hongrie]Source :
- Frontiers in immunology [ 1664-3224 ] ; 2020.
Abstract
The neonatal Fc receptor (FcRn) plays key roles in IgG and albumin homeostasis, maternal IgG transport, and antigen presentation of IgG-opsonized antigens. Previously, we reported that transgenic (Tg) mice that overexpress bovine FcRn (bFcRn) have augmented T-dependent humoral immune response with increased IgG protection, higher level of antigen-specific antibodies, greater number of antigen-specific B cells, and effective immune response even against weakly immunogenic epitopes. In this study we analyzed the diversity of the humoral immune response of bFcRn Tg mice, using a length distribution analysis (spectratyping) and next generation sequencing (NGS) of the immunoglobulin heavy chain variable regions. Our analysis showed that in response to immunization with ovalbumin or transfected cells that expressed a unique membrane protein, our Tg animals developed a more diverse plasma cell repertoire than controls, which manifested in greater numbers of different clones, and clusters with fewer highly expanded large clones, as identified by the variable region (CDR3) of the immunoglobulin heavy chain. The increased antibody diversity in Tg mice after immunization was observed at both IgM and IgG levels, indicating that the increased humoral immune diversity in Tg mice is due to a higher number of both activated, antigen-specific naïve and isotype switched B cells. We thus demonstrated that the BCR repertoire of the immunized bFcRn Tg animals is more diverse compared to wild type mice, which likely makes these Tg mice a better choice for monoclonal antibody production against challenging antigens, including the extracellular regions of cell membrane proteins.
DOI: 10.3389/fimmu.2020.01887
PubMed: 32973781
PubMed Central: PMC7472951
Affiliations:
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Szikora</name><affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation></author><author><name sortKey="Marx, Anita" sort="Marx, Anita" uniqKey="Marx A" first="Anita" last="Marx">Anita Marx</name><affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie 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Jani</name><affiliation wicri:level="1"><nlm:affiliation>ImmunoGenes Ltd., Budakeszi, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>ImmunoGenes Ltd., Budakeszi</wicri:regionArea><wicri:noRegion>Budakeszi</wicri:noRegion></affiliation></author><author><name sortKey="Pipek, Orsolya" sort="Pipek, Orsolya" uniqKey="Pipek O" first="Orsolya" last="Pipek">Orsolya Pipek</name><affiliation wicri:level="3"><nlm:affiliation>Department of Physics of Complex Systems, Institute of Physics, Eötvös Loránd University, Budapest, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Department of Physics of Complex Systems, Institute of Physics, Eötvös Loránd University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation></author><author><name sortKey="Muller, Viktor" sort="Muller, Viktor" uniqKey="Muller V" first="Viktor" last="Müller">Viktor Müller</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Systematics, Ecology and Theoretical Biology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Department of Plant Systematics, Ecology and Theoretical Biology, Institute of Biology, Eötvös Loránd University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation></author><author><name sortKey="Csabai, Istvan" sort="Csabai, Istvan" uniqKey="Csabai I" first="István" last="Csabai">István Csabai</name><affiliation wicri:level="3"><nlm:affiliation>Department of Physics of Complex Systems, Institute of Physics, Eötvös Loránd University, Budapest, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Department of Physics of Complex Systems, Institute of Physics, Eötvös Loránd University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation></author><author><name sortKey="Kacskovics, Imre" sort="Kacskovics, Imre" uniqKey="Kacskovics I" first="Imre" last="Kacskovics">Imre Kacskovics</name><affiliation wicri:level="3"><nlm:affiliation>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>ImmunoGenes Ltd., Budakeszi, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>ImmunoGenes Ltd., Budakeszi</wicri:regionArea><wicri:noRegion>Budakeszi</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in immunology</title><idno type="eISSN">1664-3224</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The neonatal Fc receptor (FcRn) plays key roles in IgG and albumin homeostasis, maternal IgG transport, and antigen presentation of IgG-opsonized antigens. Previously, we reported that transgenic (Tg) mice that overexpress bovine FcRn (bFcRn) have augmented T-dependent humoral immune response with increased IgG protection, higher level of antigen-specific antibodies, greater number of antigen-specific B cells, and effective immune response even against weakly immunogenic epitopes. In this study we analyzed the diversity of the humoral immune response of bFcRn Tg mice, using a length distribution analysis (spectratyping) and next generation sequencing (NGS) of the immunoglobulin heavy chain variable regions. Our analysis showed that in response to immunization with ovalbumin or transfected cells that expressed a unique membrane protein, our Tg animals developed a more diverse plasma cell repertoire than controls, which manifested in greater numbers of different clones, and clusters with fewer highly expanded large clones, as identified by the variable region (CDR3) of the immunoglobulin heavy chain. The increased antibody diversity in Tg mice after immunization was observed at both IgM and IgG levels, indicating that the increased humoral immune diversity in Tg mice is due to a higher number of both activated, antigen-specific naïve and isotype switched B cells. We thus demonstrated that the BCR repertoire of the immunized bFcRn Tg animals is more diverse compared to wild type mice, which likely makes these Tg mice a better choice for monoclonal antibody production against challenging antigens, including the extracellular regions of cell membrane proteins.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32973781</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>26</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1664-3224</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in immunology</Title><ISOAbbreviation>Front Immunol</ISOAbbreviation></Journal><ArticleTitle>FcRn Overexpression Expands Diversity of the Humoral Immune Response in bFcRn Transgenic Mice.</ArticleTitle><Pagination><MedlinePgn>1887</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fimmu.2020.01887</ELocationID><Abstract><AbstractText>The neonatal Fc receptor (FcRn) plays key roles in IgG and albumin homeostasis, maternal IgG transport, and antigen presentation of IgG-opsonized antigens. Previously, we reported that transgenic (Tg) mice that overexpress bovine FcRn (bFcRn) have augmented T-dependent humoral immune response with increased IgG protection, higher level of antigen-specific antibodies, greater number of antigen-specific B cells, and effective immune response even against weakly immunogenic epitopes. In this study we analyzed the diversity of the humoral immune response of bFcRn Tg mice, using a length distribution analysis (spectratyping) and next generation sequencing (NGS) of the immunoglobulin heavy chain variable regions. Our analysis showed that in response to immunization with ovalbumin or transfected cells that expressed a unique membrane protein, our Tg animals developed a more diverse plasma cell repertoire than controls, which manifested in greater numbers of different clones, and clusters with fewer highly expanded large clones, as identified by the variable region (CDR3) of the immunoglobulin heavy chain. The increased antibody diversity in Tg mice after immunization was observed at both IgM and IgG levels, indicating that the increased humoral immune diversity in Tg mice is due to a higher number of both activated, antigen-specific naïve and isotype switched B cells. We thus demonstrated that the BCR repertoire of the immunized bFcRn Tg animals is more diverse compared to wild type mice, which likely makes these Tg mice a better choice for monoclonal antibody production against challenging antigens, including the extracellular regions of cell membrane proteins.</AbstractText><CopyrightInformation>Copyright © 2020 Szikora, Marx, Jani, Pipek, Müller, Csabai and Kacskovics.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Szikora</LastName><ForeName>Bence</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marx</LastName><ForeName>Anita</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jani</LastName><ForeName>Péter K</ForeName><Initials>PK</Initials><AffiliationInfo><Affiliation>ImmunoGenes Ltd., Budakeszi, Hungary.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pipek</LastName><ForeName>Orsolya</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Physics of Complex Systems, Institute of Physics, Eötvös Loránd University, Budapest, Hungary.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>Viktor</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Department of Plant Systematics, Ecology and Theoretical Biology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Csabai</LastName><ForeName>István</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Physics of Complex Systems, Institute of Physics, Eötvös Loránd University, Budapest, Hungary.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kacskovics</LastName><ForeName>Imre</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>ImmunoGenes Ltd., Budakeszi, Hungary.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Immunol</MedlineTA><NlmUniqueID>101560960</NlmUniqueID><ISSNLinking>1664-3224</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">B cell repertoire</Keyword><Keyword MajorTopicYN="N">FcRn overexpression</Keyword><Keyword MajorTopicYN="N">humoral immune response</Keyword><Keyword MajorTopicYN="N">monoclonal antibody production</Keyword><Keyword MajorTopicYN="N">next generation sequencing</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>07</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>9</Month><Day>25</Day><Hour>6</Hour><Minute>2</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32973781</ArticleId><ArticleId IdType="doi">10.3389/fimmu.2020.01887</ArticleId><ArticleId IdType="pmc">PMC7472951</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Science. 2009 May 8;324(5928):807-10</Citation><ArticleIdList><ArticleId IdType="pubmed">19423829</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1989 Jan 12;337(6203):184-7</Citation><ArticleIdList><ArticleId IdType="pubmed">2911353</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Neurosci. 2015 Jan;18(1):75-86</Citation><ArticleIdList><ArticleId IdType="pubmed">25485758</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Immunol. 2007 Sep;7(9):715-25</Citation><ArticleIdList><ArticleId IdType="pubmed">17703228</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 1996 Oct 1;157(7):2779-88</Citation><ArticleIdList><ArticleId IdType="pubmed">8816380</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Med. 2015 Nov 20;7:121</Citation><ArticleIdList><ArticleId IdType="pubmed">26589402</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2018 Jan 10;13(1):e0190982</Citation><ArticleIdList><ArticleId IdType="pubmed">29320559</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Immunol. 2009;103:77-115</Citation><ArticleIdList><ArticleId IdType="pubmed">19755184</ArticleId></ArticleIdList></Reference><Reference><Citation>Immunol Rev. 2015 Nov;268(1):269-87</Citation><ArticleIdList><ArticleId IdType="pubmed">26497527</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1964 Sep 26;203:1352-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14207307</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2019 Feb;566(7744):393-397</Citation><ArticleIdList><ArticleId IdType="pubmed">30664748</ArticleId></ArticleIdList></Reference><Reference><Citation>MAbs. 2011 Mar-Apr;3(2):173-80</Citation><ArticleIdList><ArticleId IdType="pubmed">21239891</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2019 Oct 3;9(1):14261</Citation><ArticleIdList><ArticleId IdType="pubmed">31582818</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Nutr Soc. 1969 Mar;28(1):35-41</Citation><ArticleIdList><ArticleId IdType="pubmed">4182340</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Biol Med Model. 2014 Jul 03;11:30</Citation><ArticleIdList><ArticleId IdType="pubmed">24992938</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2016 Oct;11(10):1908-1923</Citation><ArticleIdList><ArticleId IdType="pubmed">27658009</ArticleId></ArticleIdList></Reference><Reference><Citation>J Leukoc Biol. 2019 Mar;105(3):531-538</Citation><ArticleIdList><ArticleId IdType="pubmed">30556925</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Immunol. 1985 Jul;15(7):733-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2988974</ArticleId></ArticleIdList></Reference><Reference><Citation>MAbs. 2010 Nov-Dec;2(6):594-606</Citation><ArticleIdList><ArticleId IdType="pubmed">20864805</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Immunol. 2007 Feb;44(6):1057-64</Citation><ArticleIdList><ArticleId IdType="pubmed">16930714</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2017 Mar 15;8:14714</Citation><ArticleIdList><ArticleId IdType="pubmed">28294127</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Oct 23;8(10):e76839</Citation><ArticleIdList><ArticleId IdType="pubmed">24194847</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci China Life Sci. 2020 Aug;63(8):1240-1250</Citation><ArticleIdList><ArticleId IdType="pubmed">31321668</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2011 Jan 15;186(2):959-68</Citation><ArticleIdList><ArticleId IdType="pubmed">21148035</ArticleId></ArticleIdList></Reference><Reference><Citation>Autoimmunity. 2008 Feb;41(1):80-6</Citation><ArticleIdList><ArticleId IdType="pubmed">18176868</ArticleId></ArticleIdList></Reference><Reference><Citation>Transgenic Res. 2007 Oct;16(5):613-27</Citation><ArticleIdList><ArticleId IdType="pubmed">17594529</ArticleId></ArticleIdList></Reference><Reference><Citation>EBioMedicine. 2015 Nov 24;2(12):2070-9</Citation><ArticleIdList><ArticleId IdType="pubmed">26844287</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Med. 2019 Jan;43(1):243-255</Citation><ArticleIdList><ArticleId IdType="pubmed">30365073</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol. 2015 Apr 1;194(7):3035-44</Citation><ArticleIdList><ArticleId IdType="pubmed">25740945</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol Methods. 2008 Dec 31;339(2):205-19</Citation><ArticleIdList><ArticleId IdType="pubmed">18926828</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Immunol. 2016 Feb;46(2):480-92</Citation><ArticleIdList><ArticleId IdType="pubmed">26614343</ArticleId></ArticleIdList></Reference><Reference><Citation>Ann Rheum Dis. 2017 Nov;76(11):1924-1930</Citation><ArticleIdList><ArticleId IdType="pubmed">28790026</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2014 Feb;32(2):158-68</Citation><ArticleIdList><ArticleId IdType="pubmed">24441474</ArticleId></ArticleIdList></Reference><Reference><Citation>Aging Cell. 2011 Dec;10(6):922-30</Citation><ArticleIdList><ArticleId IdType="pubmed">21726404</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Immunol. 2013 Nov 27;4:413</Citation><ArticleIdList><ArticleId IdType="pubmed">24348479</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Immunol. 2014 Oct 16;15:40</Citation><ArticleIdList><ArticleId IdType="pubmed">25318652</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Immunol. 2014 Aug 27;5:408</Citation><ArticleIdList><ArticleId IdType="pubmed">25221553</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(1):e28869</Citation><ArticleIdList><ArticleId IdType="pubmed">22247762</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Immunol. 2015 Jul 20;6:357</Citation><ArticleIdList><ArticleId IdType="pubmed">26257730</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Invest. 1972 Nov;51(11):2916-27</Citation><ArticleIdList><ArticleId IdType="pubmed">5080417</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2012;7(4):e36286</Citation><ArticleIdList><ArticleId IdType="pubmed">22558422</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Biol. 1984 Jul;99(1 Pt 2):159s-164s</Citation><ArticleIdList><ArticleId IdType="pubmed">6235233</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2014 Jun;11(6):653-5</Citation><ArticleIdList><ArticleId IdType="pubmed">24793455</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Hongrie</li></country><region><li>Hongrie centrale</li></region><settlement><li>Budapest</li></settlement></list><tree><country name="Hongrie"><region name="Hongrie centrale"><name sortKey="Szikora, Bence" sort="Szikora, Bence" uniqKey="Szikora B" first="Bence" last="Szikora">Bence Szikora</name></region><name sortKey="Csabai, Istvan" sort="Csabai, Istvan" uniqKey="Csabai I" first="István" last="Csabai">István Csabai</name><name sortKey="Jani, Peter K" sort="Jani, Peter K" uniqKey="Jani P" first="Péter K" last="Jani">Péter K. Jani</name><name sortKey="Kacskovics, Imre" sort="Kacskovics, Imre" uniqKey="Kacskovics I" first="Imre" last="Kacskovics">Imre Kacskovics</name><name sortKey="Kacskovics, Imre" sort="Kacskovics, Imre" uniqKey="Kacskovics I" first="Imre" last="Kacskovics">Imre Kacskovics</name><name sortKey="Marx, Anita" sort="Marx, Anita" uniqKey="Marx A" first="Anita" last="Marx">Anita Marx</name><name sortKey="Muller, Viktor" sort="Muller, Viktor" uniqKey="Muller V" first="Viktor" last="Müller">Viktor Müller</name><name sortKey="Pipek, Orsolya" sort="Pipek, Orsolya" uniqKey="Pipek O" first="Orsolya" last="Pipek">Orsolya Pipek</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|wiki= Bois
|area= PlantImRecepV1
|flux= Main
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|clé= pubmed:32973781
|texte= FcRn Overexpression Expands Diversity of the Humoral Immune Response in bFcRn Transgenic Mice.
}}
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| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
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